Anti-IGFBP2 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB90083-T60
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Anti-IGFBP2 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-IGFBP2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-IGFBP2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-IGFBP2 Magnetic Beads-IP Kit Product Description
The Anti-IGFBP2 magnetic Beads, conjugated with Anti-IGFBP2 antibody, are used for immuneprecipitation (IP) of IGFBP2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IGFBP2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IGFBP2 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-IGFBP2 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-IGFBP2 Antibody(90083-T60)
Immunogen
Recombinant Rhesus IGFBP-2 / IGFBP2 protein (Catalog#90083-C08H)
Species Reactivity
Cynomolgus IGFBP-2 / IGFBP2
Source
Polyclonal Rhesus Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus IGFBP-2 / IGFBP2 (rh IGFBP-2 / IGFBP2; Catalog#90083-C08H; F6YKM5; Met1-Gln324). IGFBP-2 / IGFBP2 specific IgG was purified by Cynomolgus IGFBP-2 / IGFBP2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
IGFBP2 Background Information

IGFBP-2, also known as IGFBP2, is a insulin-like growth factor-binding protein (IGFBP). IGFBPs prolong the half-life of the IGFs , control bioavailability, activity, and distribution of insulin-like growth factor (IGF) through high-affinity IGFBP/IGF complexes. Six high-affinity IGF-binding proteins (IGFBP-1 to -6) have been identified. The six IGFBPs are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP-2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP-2 in tumorigenesis. It contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. It inhibits IGF-mediated growth and developmental rates.

Full Name
insulin-like growth factor binding protein 2, 36kDa
References
  • Han VK, et al. (1996) The expression of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) genes in the human placenta and membranes: evidence for IGF-IGFBP interactions at the feto-maternal interface. J Clin Endocrinol Metab. 81(7): 2680-93.
  • Binkert C, et al. (1989) Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein (IGFBP-2) . EMBO J. 8(9):2497-502.
  • Wolf E, et al. (2000) Effects of IGFBP-2 overexpression in vitro and in vivo. Pediatr Nephrol. 14 (7):572-8.
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