Anti-interferon-alpha Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80205-T60
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Anti-interferon-alpha Magnetic Beads-IP Kit Product Components
Components Storage
Anti-interferon-alpha Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-interferon-alpha magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-interferon-alpha Magnetic Beads-IP Kit Product Description
The Anti-interferon-alpha magnetic Beads, conjugated with Anti-interferon-alpha antibody, are used for immuneprecipitation (IP) of interferon-alpha proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing interferon-alpha proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound interferon-alpha proteins are dissociated from the magnetic beads using an elution buffer.
Anti-interferon-alpha Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-interferon-alpha Antibody(80205-T60)
Immunogen
Recombinant Rat IFNA5 / IFNaG protein (Catalog#80205-R08H)
Species Reactivity
Rat IFNA5 / IFNaG
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat IFNA5 / IFNaG (rh IFNA5 / IFNaG; Catalog#80205-R08H; XP_001076062.1; Met1-Glu189). IFNA5 / IFNaG specific IgG was purified by Rat IFNA5 / IFNaG affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-interferon-alpha Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-IFNA5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
interferon-alpha Background Information

Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

Full Name
interferon, alpha 5
References
  • Lau JY, et al. (1993) Discrepancy between biochemical and virological responses to interferon-alpha in chronic hepatitis C. Lancet. 342(8881): 1208-9.
  • Kessler DS, et al. (1990) Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator. Genes Dev. 4(10): 1753-65.
  • Gutterman JU. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A. 91(4): 1198-205.
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