Anti-Galectin-1 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80248-T52
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Anti-Galectin-1 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-Galectin-1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-Galectin-1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Galectin-1 Magnetic Beads-IP Kit Product Description
The Anti-Galectin-1 magnetic Beads, conjugated with Anti-Galectin-1 antibody, are used for immuneprecipitation (IP) of Galectin-1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Galectin-1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Galectin-1 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-Galectin-1 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-Galectin-1 Antibody(80248-T52)
Immunogen
Recombinant Rat Galectin-1 / LGALS1 Protein (Catalog#80248-RNAE)
Species Reactivity
Rat Galectin-1 / LGALS1
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat Galectin-1 / LGALS1 (rR Galectin-1 / LGALS1; Catalog#80248-RNAE; P11762; Ala2-Glu135). Galectin-1 / LGALS1 specific IgG was purified by Rat Galectin-1 / LGALS1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Galectin-1 Background Information

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.

Full Name
lectin, galactoside-binding, soluble, 1
References
  • Gaudet AD, et al. (2005) Expression and functions of galectin-1 in sensory and motoneurons. Curr Drug Targets. 6(4): 419-25.
  • Kadoya T, et al. (2006) Structural and functional studies of galectin-1: a novel axonal regeneration-promoting activity for oxidized galectin-1. Curr Drug Targets. 6(4): 375-83.
  • Camby I, et al. (2006) Galectin-1: a small protein with major functions. Glycobiology. 16(11): 137R-157R.
  • Salatino M, et al. (2008) Galectin-1 as a potential therapeutic target in autoimmune disorders and cancer. Expert Opin Biol Ther. 8(1): 45-57.
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