Anti-Ephrin-B1 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80106-T52

More Discounts Available
Contact us:
All Ephrin-B1 Reagents
CRO Services
Product recommended

All Ephrin-B1 Reagents
Anti-Ephrin-B1 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-Ephrin-B1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-Ephrin-B1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Ephrin-B1 Magnetic Beads-IP Kit Product Description
The Anti-Ephrin-B1 magnetic Beads, conjugated with Anti-Ephrin-B1 antibody, are used for immuneprecipitation (IP) of Ephrin-B1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Ephrin-B1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Ephrin-B1 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-Ephrin-B1 Magnetic Beads-IP Kit Antibody Information
Anti-Ephrin-B1 Antibody(80106-T52)
Recombinant Rat Ephrin-B1 / EFNB1 protein (Catalog#80106-R08H)
Species Reactivity
Rat Ephrin-B1 / EFNB1
Polyclonal Rat Rabbit IgG
Produced in rabbits immunized with purified, recombinant Rat Ephrin-B1 / EFNB1 (rh Ephrin-B1 / EFNB1; Catalog#80106-R08H; P52796; Met1-Thr229). Ephrin-B1 / EFNB1 specific IgG was purified by Rat Ephrin-B1 / EFNB1 affinity chromatography.
Immunoprecipitation (IP), Minimum Protein Purification
Ephrin-B1 Background Information

Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

Full Name
  • Davy A, et al. (2004) Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev. 18(5): 572-83.
  • Compagni A, et al. (2003) Control of skeletal patterning by ephrinB1-EphB interactions. Dev Cell. 5(2): 217-30.
  • Wieland I, et al. (2004) Mutations of the ephrin-B1 gene cause craniofrontonasal syndrome. Am J Hum Genet. 74(6): 1209-15.
  • info info
    添加购物车成功! 添加购物车失败!