Anti-ENO1 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80578-T52
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Anti-ENO1 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-ENO1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-ENO1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-ENO1 Magnetic Beads-IP Kit Product Description
The Anti-ENO1 magnetic Beads, conjugated with Anti-ENO1 antibody, are used for immuneprecipitation (IP) of ENO1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ENO1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ENO1 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-ENO1 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-ENO1 Antibody(80578-T52)
Immunogen
Recombinant Rat ENO1 / Enolase 1 / alpha-enolase protein (Catalog#80578-R07E)
Species Reactivity
Rat ENO1 / Enolase 1 / alpha-e
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat ENO1 / Enolase 1 / alpha-enolase (rh ENO1 / Enolase 1 / alpha-enolase; Catalog#80578-R07E; NP_036686.2; Met1-Lys434). ENO1 / Enolase 1 / alpha-enolase specific IgG was purified by Rat ENO1 / Enolase 1 / alpha-enolase affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ENO1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-Eno-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ENO1 Background Information
Full Name
enolase 1, (alpha)
References
  • Capello M, et al. (2011) a-Enolase: a promising therapeutic and diagnostic tumor target. FEBS J. 278(7): 1064-74.
  • Kang HJ, et al. (2008) Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme. Acta Crystallogr D Biol Crystallogr. 64(Pt 6): 651-7.
  • Lopez-Alemany R, et al. (2005) Alpha-enolase plasminogen receptor in myogenesis. Front Biosci. 10: 30-6.
  • Ejeskdr K, et al. (2005) Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death. BMC Cancer. 5: 161.
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