Anti-EGFR Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80100-T52
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Anti-EGFR Magnetic Beads-IP Kit Product Components
Components Storage
Anti-EGFR Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-EGFR magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-EGFR Magnetic Beads-IP Kit Product Description
The Anti-EGFR magnetic Beads, conjugated with Anti-EGFR antibody, are used for immuneprecipitation (IP) of EGFR proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing EGFR proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound EGFR proteins are dissociated from the magnetic beads using an elution buffer.
Anti-EGFR Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-EGFR Antibody(80100-T52)
Immunogen
Recombinant Rat EGFR / HER1 / ErbB1 protein (Catalog#80100-R08H)
Species Reactivity
Rat EGFR / HER1 / ErbB1
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#80100-R08H; E7CXR8; Met1-Ser646). EGFR / HER1 / ErbB1 specific IgG was purified by Rat EGFR / HER1 / ErbB1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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