Anti-CD48 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80286-T52
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Anti-CD48 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-CD48 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-CD48 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD48 Magnetic Beads-IP Kit Product Description
The Anti-CD48 magnetic Beads, conjugated with Anti-CD48 antibody, are used for immuneprecipitation (IP) of CD48 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD48 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD48 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-CD48 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-CD48 Antibody(80286-T52)
Immunogen
Recombinant Rat CD48 / SLAMF2 protein (Catalog#80286-R08H)
Species Reactivity
Rat CD48 / SLAMF2
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat CD48 / SLAMF2 (rh CD48 / SLAMF2; Catalog#80286-R08H; P10252; Met1-Arg216). CD48 / SLAMF2 specific IgG was purified by Rat CD48 / SLAMF2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
CD48 Background Information

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD48 molecule
References
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
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