Anti-B7-H3 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80380-T52
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Anti-B7-H3 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-B7-H3 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-B7-H3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-B7-H3 Magnetic Beads-IP Kit Product Description
The Anti-B7-H3 magnetic Beads, conjugated with Anti-B7-H3 antibody, are used for immuneprecipitation (IP) of B7-H3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing B7-H3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound B7-H3 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-B7-H3 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-B7-H3 Antibody(80380-T52)
Immunogen
Recombinant Rat B7-H3 / CD276 protein (Catalog#80380-R08H)
Species Reactivity
Rat B7-H3 / CD276
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat B7-H3 / CD276 (rh B7-H3 / CD276; Catalog#80380-R08H; Q7TPB4; Met1-Phe244). B7-H3 / CD276 specific IgG was purified by Rat B7-H3 / CD276 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
B7-H3 Background Information

B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD276 molecule
References
  • Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.
  • Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.
  • Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.
  • Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.
  • Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.
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