Anti-Beta-2 microglobulin Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB90152-T52
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Anti-Beta-2 microglobulin Magnetic Beads-IP Kit Product Components
Components Storage
Anti-Beta-2 microglobulin Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-Beta-2 microglobulin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Beta-2 microglobulin Magnetic Beads-IP Kit Product Description
The Anti-Beta-2 microglobulin magnetic Beads, conjugated with Anti-Beta-2 microglobulin antibody, are used for immuneprecipitation (IP) of Beta-2 microglobulin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Beta-2 microglobulin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Beta-2 microglobulin proteins are dissociated from the magnetic beads using an elution buffer.
Anti-Beta-2 microglobulin Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-Beta-2 microglobulin Antibody(90152-T52)
Immunogen
Recombinant Cynomolgus B2M / beta-2 microglobulin Protein (Catalog#90152-C08H)
Species Reactivity
Cynomolgus B2M / beta-2 microg
Source
Polyclonal Cynomolgus Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus B2M / beta-2 microglobulin (Catalog#90152-C08H; Q6V7J5; Met1-Met119). B2M / beta-2 microglobulin specific IgG was purified by Cynomolgus B2M / beta-2 microglobulin affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Beta-2 microglobulin Background Information

B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M(β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M(β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M(β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to theα3 chain. Unlikeα3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M(β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M(β2 microglobulin) can indicate non-progression of HIV.

Full Name
beta-2 microglobulin
References
  • Poulik MD, et al. (1979) Beta 2-Microglobulin: methods and clinical applications. CRC Ctit Rev Clin Lab Sci. 10(3): 225-45.
  • Poulik MD, et al. (1975) Beta2-Microglobulins. Contemp Top Mol Immunol. 4: 157-204.
  • Berggard I. (1976) Beta2-Microglobulins: isolation, properties, and distribution. Fed Proc. 35(5): 1167-70.
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