Anti-Angiopoietin-2 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB90026-T52

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Anti-Angiopoietin-2 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-Angiopoietin-2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-Angiopoietin-2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Angiopoietin-2 Magnetic Beads-IP Kit Product Description
The Anti-Angiopoietin-2 magnetic Beads, conjugated with Anti-Angiopoietin-2 antibody, are used for immuneprecipitation (IP) of Angiopoietin-2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Angiopoietin-2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Angiopoietin-2 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-Angiopoietin-2 Magnetic Beads-IP Kit Antibody Information
Anti-Angiopoietin-2 Antibody(90026-T52)
Recombinant Cynomolgus Angiopoietin-2 / ANG2 protein (Catalog#90026-C07H)
Species Reactivity
Cynomolgus Angiopoietin-2 / AN
Polyclonal Cynomolgus Rabbit IgG
Produced in rabbits immunized with purified, recombinant Cynomolgus Angiopoietin-2 / ANG2 (rh Angiopoietin-2 / ANG2; Catalog#90026-C07H; XP_005562585.1; Lys274-Phe495). Angiopoietin-2 / ANG2 specific IgG was purified by Cynomolgus Angiopoietin-2 / ANG2 affinity chromatography.
Immunoprecipitation (IP), Minimum Protein Purification
Angiopoietin-2 Background Information

Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.

Full Name
angiopoietin 2
  • Thomas M, et al. (2009) The role of the Angiopoietins in vascular morphogenesis. Angiogenesis. 12(2): 125-37.
  • Hu B, et al. (2009) Angiopoietin-2: development of inhibitors for cancer therapy. Curr Oncol Rep. 11(2): 111-6.
  • Fiedler U, et al. (2006) Angiopoietins: a link between angiogenesis and inflammation. Trends Immunol. 27: 552-8.
  • Escobar E, et al. (2004) Angiotensin II, cell proliferation and angiogenesis regulator: biologic and therapeutic implications in cancer. Curr Vasc Pharmacol. 2(4): 385-99.
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