All IL-18 reagents are produced in house and quality controlled, including 13 IL-18 Antibody, 4 IL-18 ELISA, 65 IL-18 Gene, 2 IL-18 IPKit, 4 IL-18 Protein, 3 IL-18 qPCR. All IL-18 reagents are ready to use.
Recombinant IL-18 proteins are expressed by E. coli with fusion tags as Native, N-cleavage, N-His, N-GST.
IL-18antibodies are validated with different applications, which are ELISA, ELISA(Cap), WB, IP, ELISA(Det).
IL-18cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each IL-18 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
IL-18ELISA Kit are quality controlled by 8 internation QC standard which guarantee every ELISA Kit with high quality.
Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.