Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody

Cat: 11049-R338
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Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody (Rabbit Monoclonal antibody) General Information
Product name
Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody
Validated applications
ELISA(Cap),Microneutralizaiton(MN) (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
RSV RSV Fusion
Has cross-reactivity with recomibnant F protein of A type of long strain (Catalog # 40039-V08B) , A2 strain (Catalog # 11049-V08B) , RSS2 strain (Catalog # 40037-V08B) and B type of
Immunogen
Recombinant Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F protein (Catalog#11049-V08B)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F (Catalog#11049-V08B; AAB59858.1; Met1-Thr529) and was produced using recombinant antibody technology.
Source
Monoclonal Rabbit IgG Clone #R338
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Endotoxin
Please contact us for more information.
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA(Cap) 0.5-4 µg/ml This antibody will detect Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F in ELISA pair set (Catalog: # SEK11049). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 11049-R302).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody (Rabbit Monoclonal antibody) Images
RSV RSV F Microneutralizaiton (MN) 5570
The neutralization activity of RSV F protein antibody is Measured by Microneutralization test in vitro. The cytopathic effect of VERO cells induced by 100 pfu of RSV A-A2 strain is neutralized by increasing concentrations of anti RSV Monoclonal Antibody (Catalog # 11049-R338). The neutralizing titer (IC50) of antibody is 0.6-2.3 μg/mL.
Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody Alternative Names
Anti-F Antibody;Anti-HRSVgp08 Antibody
RSV Fusion Background Information

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F0), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.

References
  • Martin-Gallardo A. et al., 1993, J Gen Virol. 74 (3): 453-8.
  • Jose A M. et al., 1997, J Gen Virol. 78: 2411-8.
  • Feldman SA. et al., 1999, J Virol. 73 (8): 6610-7.
  • Zlateva K.T. et al., 2004, J Virol. 78 (9): 4675-83.
  • Trento A. et al., 2006, J Virol. 80 (2): 975-84.
  • Branigan P J. et al., 2006, J Gen Virol. 87 (2): 395-8.
  • Eckardt-Michel J. et al., 2008, J. Virol. 82: 3236-49.
  • Product Description Host Clonality Application Catalog# (PDF)
    Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Antibody, Rabbit MAb Rabbit Monoclonal ELISA(Det) 11049-R302
    Human respiratory syncytial virus (RSV) Fusion Glycoprotein / RSV-F Antibody, Rabbit MAb Rabbit Monoclonal ELISA,IHC-P,FCM,ICC/IF,IF,IP 11049-R009
    info info
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