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人類呼吸道合胞病毒 hRSV Fusion glycoprotein / RSV-F 中和抗體

  • Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody
產品資料評論相關產品實驗方法
RSV RSV-F 抗體產品資訊
免疫原:Recombinant Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F protein (Catalog#11049-V08B)
Clone ID:R338
Ig類型:Rabbit IgG
濃度:
內毒素:Please contact us for more information.
緩衝液:0.2 μm filtered solution in PBS
抗體製備方法:This antibody was obtained from a rabbit immunized with purified, recombinant Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F (Catalog#11049-V08B; AAB59858.1; Met1-Thr529) and was produced using recombinant antibody technology.
RSV RSV-F Antibody Neutralization Application Image
Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody
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Caption:
The neutralization activity of RSV F protein antibody is Measured by Microneutralization test in vitro. The infection of VERO cells induced by 140-160 pfu of RSV A-A2 strain is neutralized by increasing concentrations of anti RSV Monoclonal Antibody (Catalog: 11049-R338). The neutralizing titer (IC50) of antibody is 0.6-2.3 µg/mL.
Negative Control: VERO cells cells were infected with 140-160 pfu of RSV A-A2 strain;
Positive Control: The RSV (140-160 pfu A-A2 strain) infection in VERO cells was inhibited by serial dilutions of positive Neutralizing Antibody;
RSV-F R338: The RSV (140-160 pfu A-A2 strain) infection in VERO cells was inhibited by serial dilutions of RSV-F R338.
Plaque numbers represent number of Plaque-forming units with or without antibody.

Other RSV-F Antibody Products
Immunochemical staining of human CD40 in human tonsil with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
RSV F 研究背景

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F0), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.

RSV RSV F 參考資料
  • Martin-Gallardo A. et al., 1993, J Gen Virol. 74 (3): 453-8.
  • Jose A M. et al., 1997, J Gen Virol. 78: 2411-8.
  • Feldman SA. et al., 1999, J Virol. 73 (8): 6610-7.
  • Zlateva K.T. et al., 2004, J Virol. 78 (9): 4675-83.
  • Trento A. et al., 2006, J Virol. 80 (2): 975-84.
  • Branigan P J. et al., 2006, J Gen Virol. 87 (2): 395-8.
  • Eckardt-Michel J. et al., 2008, J. Virol. 82: 3236-49.
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    貨號: 11049-R338-500
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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