输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!
Text Size:AAA

人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His 標籤

產品資料評論相關產品實驗方法
RSV RSV-F cDNA Clone產品資訊
註冊碼:
cDNA基因長度:1725bp
cDNA基因描述:Full length Clone DNA of Human RSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F with N terminal His tag.
基因別名:F, HRSVgp08
分子種屬:RSV
載體:pCMV3-SP-N-His
Plasmid:pCMV3-His-RSV-F(A-RSS2)
限制性酶切位點:KpnI + XbaI (6kb + 1.74kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with P11209.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
RSV RSV-F Gene Plasmid Map
Human respiratory syncytial virus (RSV) (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His 標籤 on other vectors
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40037-ACGNT$12500
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP 標籤VG40037-ACRNT$12500
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), C-Flag 標籤VG40037-CFNT$11410
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, C-His 標籤VG40037-CHNT$11410
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, C-Myc 標籤VG40037-CMNT$11410
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, C-HA 標籤VG40037-CYNT$11410
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized)VG40037-GNT$4170
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-Flag 標籤VG40037-NFNT$11410
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His 標籤VG40037-NHNT$11410
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40037-NMNT$11410
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-HA 標籤VG40037-NYNT$11410
人類呼吸道合胞病毒 hRSV (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized)VG40037-UTNT$11410
 點擊瞭解更多載體資訊
Product nameProduct name
研究背景

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F0), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.

參考資料
  • Martin-Gallardo A. et al., 1993, J Gen Virol. 74 (3): 453-8.
  • Jose A M. et al., 1997, J Gen Virol. 78: 2411-8.
  • Feldman SA. et al., 1999, J Virol. 73 (8): 6610-7.
  • Zlateva K.T. et al., 2004, J Virol. 78 (9): 4675-83.
  • Trento A. et al., 2006, J Virol. 80 (2): 975-84.
  • Branigan P J. et al., 2006, J Gen Virol. 87 (2): 395-8.
  • Eckardt-Michel J. et al., 2008, J. Virol. 82: 3236-49.
  • Size / Price
    貨號: VG40037-NH
    目錄價: 
    單價:      (You Save: )
    規格:
    數量:+-
    庫存In Stock
    大包裝查詢加入購物車
    Contact Us
    產品圖片
    • Human respiratory syncytial virus (RSV) (subtype A, strain RSS-2) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His tag
    • Rhesus CD3d/CD3 delta Gene Plasmid Map 5615
      您曾流覽過的產品
        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”