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人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His 標籤

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    RSV RSV-F cDNA Clone產品資訊
    註冊碼:
    cDNA基因長度:1725bp
    cDNA基因描述:Full length Clone DNA of Human RSV (subtype A, strain Long) Fusion glycoprotein / RSV-F with N terminal His tag.
    基因別名:F, HRSVgp08
    分子種屬:RSV
    載體:pCMV3-SP-N-His
    Plasmid:
    限制性酶切位點:
    Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
    序列資訊:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with P12568.1.
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
    His Tag Info

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

    Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His 標籤 on other vectors
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40039-ACGNT$12500
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP 標籤VG40039-ACRNT$12500
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), C-Flag 標籤VG40039-CFNT$11410
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, C-His 標籤VG40039-CHNT$11410
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, C-Myc 標籤VG40039-CMNT$11410
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, C-HA 標籤VG40039-CYNT$11410
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized)VG40039-GNT$4170
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-Flag 標籤VG40039-NFNT$11410
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-His 標籤VG40039-NHNT$11410
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40039-NMNT$11410
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F (Codon Optimized) ORF mammalian expression plasmid, N-HA 標籤VG40039-NYNT$11410
    人類呼吸道合胞病毒 hRSV (subtype A, strain Long) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized)VG40039-UTNT$11410
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    研究背景

    Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F0), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.

    參考資料
  • Martin-Gallardo A. et al., 1993, J Gen Virol. 74 (3): 453-8.
  • Jose A M. et al., 1997, J Gen Virol. 78: 2411-8.
  • Feldman SA. et al., 1999, J Virol. 73 (8): 6610-7.
  • Zlateva K.T. et al., 2004, J Virol. 78 (9): 4675-83.
  • Trento A. et al., 2006, J Virol. 80 (2): 975-84.
  • Branigan P J. et al., 2006, J Gen Virol. 87 (2): 395-8.
  • Eckardt-Michel J. et al., 2008, J. Virol. 82: 3236-49.
  • Size / Price
    貨號: VG40039-NH
    目錄價: 
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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