( We provide with CXCL8 qPCR primers for gene expression analysis, HP100179 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10098-ACG|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10098-ACR|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10098-CF|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10098-CH|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10098-CM|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10098-CY|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF(克隆載體)||HG10098-M|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10098-NF|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10098-NH|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10098-NM|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10098-NY|
|人 IL-8/Interleukin-8/CXCL8 基因全長cDNA ORF克隆 (表達載體)||HG10098-UT|
Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.