|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG12163-ACG|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG12163-ACR|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG12163-CF|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG12163-CH|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG12163-CM|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG12163-CY|
|人 VSIG4 基因全長cDNA ORF(克隆載體)||HG12163-G|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG12163-G-F|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG12163-NF|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG12163-NH|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG12163-NM|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG12163-NY|
|人 VSIG4 基因全長cDNA ORF克隆 (表達載體)||HG12163-UT|
VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 280 amino acid precursor that contains a signal sequence, an V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 80% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.