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人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤

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Human USH1C cDNA Clone產品資訊
註冊碼:NM_005709.2
cDNA基因長度:1659bp
cDNA基因描述:Full length Clone DNA of Homo sapiens Usher syndrome 1C (autosomal recessive, severe), transcript variant 1 with C terminal Myc tag.
基因別名:PDZ73, AIE-75, DFNB18, PDZ-45, PDZ-73, NY-CO-37, NY-CO-38, ush1cpst, PDZ-73/NY-CO-38
分子種屬:Human
載體:pCMV3-C-Myc
Plasmid:
限制性酶切位點:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤 on other vectors
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10613-ACGNT$8880
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10613-ACRNT$8880
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG10613-ANGNT$8880
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG10613-ANRNT$8880
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10613-CFNT$7790
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10613-CHNT$7790
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10613-CMNT$7790
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10613-CYNT$7790
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF(克隆載體)HG10613-MNT$2720
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10613-M-FNT$7790
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10613-NFNT$7790
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10613-NHNT$7790
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10613-NMNT$7790
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10613-NYNT$7790
人 USH1C/Harmonin transcript variant 1 基因全長cDNA ORF克隆 (表達載體)HG10613-UTNT$7790
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研究背景

Harmonin, also known as Antigen NY-CO-38 / NY-CO-37, Autoimmune enteropathy-related antigen AIE-75, Protein PDZ-73, Renal carcinoma antigen NY-REN-3, Usher syndrome type-1C protein and USH1C, is a protein which is expressed in small intestine, colon, kidney, eye and weakly in pancreas. USH1C is expressed also in vestibule of the inner ear. USH1C contains 3 PDZ (DHR) domains. USH1C may be involved in protein-protein interaction. Defects in USH1C are the cause of Usher syndrome type 1C (USH1C), also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). Defects in USH1C are also the cause of deafness autosomal recessive type 18 (DFNB18) which is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

參考資料
  • Verpy, E. et al., 2000, Nat Genet. 26 (1):51-5.
  • Weil D., et al., 2003, Hum. Mol. Genet. 12:463-471.
  • Reiners,J. et al., 2005, Hum Mol Genet. 14 (24):3933-43.
  • Yan,D. et al., 2006, Mol Biol. 357 (3):755-64.
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