输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!

快速訂購

Text Size:AAA

人 TRIM63 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤

產品資料評論相關產品實驗方法
Human TRIM63 cDNA Clone產品資訊
註冊碼:NM_032588.3
cDNA基因長度:1062bp
cDNA基因描述:Full length Clone DNA of Homo sapiens tripartite motif containing 63, E3 ubiquitin protein ligase with C terminal Myc tag.
基因別名:IRF, SMRZ, MURF1, MURF2, RNF28, FLJ32380, TRIM63
分子種屬:Human
載體:pCMV3-C-Myc
Plasmid:pCMV3-TRIM63-Myc
限制性酶切位點:KpnI + XbaI (6kb + 1.11kb)
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human TRIM63 Gene Plasmid Map
Human TRIM63 ORF mammalian expression plasmid, C-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Product nameProduct name
Size / Price
貨號: HG14146-CM
目錄價: 
單價:      (You Save: )
規格:
數量:+-
庫存In Stock
大包裝查詢加入購物車
Contact Us
產品圖片
  • Human TRIM63 ORF mammalian expression plasmid, C-Myc tag
  • other Green fluorescent protein / GFP Gene Plasmid Map 5611
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”