TNF-alpha qPCR Primer Pairs, Human

Cat: HP100592
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TNF-alpha qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
NCBI RefSeq:
PCR Size:
132bp
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
TNF-alpha qPCR Primer Pairs, Human Alternative Names
DIF qPCR Primer Pairs, Human;TNF-alpha qPCR Primer Pairs, Human;TNFA qPCR Primer Pairs, Human;TNFSF2 qPCR Primer Pairs, Human
TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
tumor necrosis factor
Research Areas
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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    Product Name Catalog#(PDF)
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    TNF-alpha Lentiviral cDNA ORF Clone, Ferret, C-GFPSpark® tag FG60002-ACGLN
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    TNF-alpha cDNA ORF Clone, Ferret, C-DDK (Flag®) tag FG60002-CF
    TNF-alpha cDNA ORF Clone, Ferret, N-DDK (Flag®) tag FG60002-NF
    TNF-alpha cDNA ORF Clone, Ferret, C-His tag FG60002-CH
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    TNF-alpha cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90018-ACG
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    TNF-alpha cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90018-CF
    TNF-alpha cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90018-NF
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    TNF-alpha cDNA ORF Clone in Cloning Vector, Rat RG80045-M
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    Product Name Catalog#(PDF)
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    TNF-alpha cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50349-ACR
    TNF-alpha cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50349-CF
    TNF-alpha cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50349-NF
    TNF-alpha cDNA ORF Clone, Mouse, C-His tag MG50349-CH
    TNF-alpha cDNA ORF Clone, Mouse, N-His tag MG50349-NH
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    Product Name Catalog#(PDF)
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    TNF-alpha Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10602-ACGLN
    TNF-alpha cDNA ORF Clone, Human, C-OFPSpark® tag HG10602-ACR
    TNF-alpha cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10602-CF
    TNF-alpha cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10602-NF
    TNF-alpha cDNA ORF Clone, Human, C-His tag HG10602-CH
    TNF-alpha cDNA ORF Clone, Human, N-His tag HG10602-NH
    TNF-alpha cDNA ORF Clone, Human, C-HA tag HG10602-CY
    TNF-alpha cDNA ORF Clone, Human, N-HA tag HG10602-NY
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    TNF-alpha cDNA ORF Clone, Human, N-Myc tag HG10602-NM
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