TNF-alpha Protein, Human, Recombinant

Cat: GMP-10602-HNAE
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All TNF-alpha Reagents
TNF-alpha Protein, Human, Recombinant Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 10 EU per mg protein as determined by the LAL method.
Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The specific activity of this protein is approximately 7.6x10E4 IU/μg.
Protein Construction
A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val77-Leu233) was expressed with an initial Met at the N-terminus.
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TNF-alpha Protein, Human, Recombinant in SDS-PAGE
Human TNF-alpha/TNFA/TNFSF2 Protein 14104
TNF-alpha Protein, Human, Recombinant Alternative Names
DIF Protein, Human;TNF-alpha Protein, Human;TNFA Protein, Human;TNFSF2 Protein, Human
TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
tumor necrosis factor
Research Areas
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Other TNF-alpha Proteins

    Canine TNF-alpha Proteins

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    TNF-alpha Protein, Canine, Recombinant 70003-DNAE

    Cynomolgus TNF-alpha Proteins

    Product Name Cat.No.
    TNF-alpha Protein, Cynomolgus, Recombinant 90330-CNAE
    TNF-alpha Protein, Cynomolgus, Recombinant 90018-CNAE

    Ferret TNF-alpha Proteins

    Product Name Cat.No.
    TNF-alpha Protein, Ferret, Recombinant 60002-FNAE

    Rat TNF-alpha Proteins

    Product Name Cat.No.
    TNF-alpha Protein, Rat, Recombinant 80045-RNAE

    Mouse TNF-alpha Proteins

    Product Name Cat.No.
    TNF-alpha Protein, Mouse, Recombinant 50349-MNAE
    TNF-alpha Protein, Mouse, Recombinant, Biotinylated 50349-MNAE-B

    Human TNF-alpha Proteins

    Product Name Cat.No.
    TNF-alpha Protein, Human, Recombinant (Fc Tag) 10602-H01H
    TNF-alpha Protein, Human, Recombinant 10602-HNAE
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