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人 TCF4/E2-2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤

  • Cynomolgus LOC101926731 Gene Plasmid Map 5628
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 TCF4 cDNA Clone產品資訊
註冊碼:NM_003199
cDNA基因長度:2058 bp
cDNA基因描述:Full length Clone DNA of Homo sapiens transcription factor 4 with N terminal HA tag.
基因別名:bHLHb19,E2-2,ITF-2,ITF2,PTHS,SEF-2,SEF2,SEF2-1,SEF2-1A,SEF2-1B,SEF2-1D,TCF-4
分子種屬:Human
載體:pCMV3-N-HA
Plasmid:pCMV3-HA-TCF4
限制性酶切位點:KpnI + XbaI(6kb+2.06kb)
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with TCF4 qPCR primers for gene expression analysis, HP101624 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

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