( We provide with SPN qPCR primers for gene expression analysis, HP101730 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG13108-ACG|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG13108-ACR|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG13108-CF|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG13108-CH|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG13108-CM|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG13108-CY|
|人 SPN/CD43 基因全長cDNA ORF(克隆載體)||HG13108-G|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG13108-NF|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG13108-NH|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG13108-NM|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG13108-NY|
|人 SPN/CD43 基因全長cDNA ORF克隆 (表達載體)||HG13108-UT|
CD43 is an abundantly expressed molecule on the T-cell surface that shows distinct localization to the migrating T-cell uropod and the distal pole complex (DPC) opposite the immunological synapse via association with the ezrin-radixin-moesin (ERM) family of actin regulatory proteins. CD43 has a 235-amino acid (aa) extracellular domain, a 23-aa transmembrane domain, and a 123-aa cytoplasmic domain, all encoded by a single exon. The intracytoplasmic region of the protein is necessary to transduce signals; it is rich in potentially phosphorylable threonines and serines but lacks tyrosine residues as well as catalytic activity. CD43 engagement on human peripheral blood T cells and monocytes leads to cell activation and proliferation through the generation of second messengers such as diacylglycerol and inositol phosphates, protein kinase C (PKC) activation and Ca2+ mobilization. In addition, CD43 ligation on human T cells induces the association of CD43 with Src family kinases, presumably through the interaction of their Src homology 3 domain with a proline-rich region of the CD43 intracytoplasmic tail. This molecule has been implicated in T cell activation, enhancing T cell response to allogeneic or mitogenic stimulation and CD43-specific signals have been reported to be sufficient to activate T cells in the absence of T cell receptor (TCR) engagement. In summary, CD43 regulates multiple T-cell functions, including T-cell activation, proliferation, apoptosis, and migration.