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人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤

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Human SPG21 cDNA Clone產品資訊
註冊碼:NM_016630.3
cDNA基因長度:927bp
cDNA基因描述:Full length Clone DNA of Homo sapiens spastic paraplegia 21 (autosomal recessive, Mast syndrome), transcript variant 1 with N terminal HA tag.
基因別名:MAST, ACP33, GL010, BM-019, MASPARDIN
分子種屬:Human
載體:pCMV3-N-HA
Plasmid:
限制性酶切位點:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤 on other vectors
人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10522-ACGNT$8151
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人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG10522-ANRNT$8151
人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10522-CFNT$7064
人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10522-CHNT$7064
人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10522-CMNT$7064
人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10522-CYNT$7064
人 SPG21 transcript variant 1 基因全長cDNA ORF(克隆載體)HG10522-MNT$2717
人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10522-M-FNT$7064
人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10522-NFNT$7064
人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10522-NHNT$7064
人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10522-NMNT$7064
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人 SPG21 transcript variant 1 基因全長cDNA ORF克隆 (表達載體)HG10522-UTNT$7064
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研究背景

Spastic paraplegia 21 (SPG21), also known as acid Cluster Protein 33 (ACP33) and Mast syndrome protein, is a member of the AB hydrolase superfamily. Human SPG21 is a 308 amino acid residue protein widely expressed in all tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. SPG21 binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation via the noncatalytic alpha/beta hydrolase fold domain. SPG21 thus is proposed to play a role as a negative regulatory factor in CD4-dependent T-cell activation of CD4. Defects in SPG21 are the cause of spastic paraplegia autosomal recessive type 21, also known as Mast syndrome, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. SPG21 is also associated with dementia and other central nervous system abnormalities.

參考資料
  • Zeitlmann L. et al., 2001, J Biol Chem. 276: 9123-32.
  • Simpson M. A. et al., 2003, Am J Hum Genet. 73: 1147-156.
  • Ota T. et al., 2004, Nat. Genet.36: 40-45.
  • Kedmi M. et al., 2007, Physiol Genomics. 28: 213-22.
  • Hanna M. C. et al., 2009, Neurogenetics.10: 217-28.
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    貨號: HG10522-NY
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