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人 SMOC-1 基因全長cDNA ORF克隆 (表達載體)

  • other Green fluorescent protein / GFP Gene Plasmid Map 5613
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 SMOC1 cDNA Clone產品資訊
註冊碼:NM_001034852.1
cDNA基因長度:1308bp
cDNA基因描述:Full length Clone DNA of Homo sapiens SPARC related modular calcium binding 1.
基因別名:SMOC1
分子種屬:Human
載體:pCMV3-untagged
Plasmid:pCMV3-SMOC1
限制性酶切位點:KpnI + XbaI(6kb+1.31kb)
Tag序列:
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 126G/A,702C/T not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with SMOC1 qPCR primers for gene expression analysis, HP101562 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Product nameProduct name
研究背景

SPARC-related modular calcium-binding protein 1, also known as secreted modular calcium-binding protein 1 and SMOC1, is a member of the SPARC family. SMOC1 is widely expressed in many tissues with a strongest signal in ovary. It contains two EF-hand domains, one Kazal-like domain and two thyroglobulin type-1 domains. Extracellular matrix proteins have been implicated in the regulation of osteoblast differentiation of bone marrow derived mesenchymal stem cells (BMSCs) through paracrine or autocrine mechanisms. SMOC1 is a regulator of osteoblast differentiation of BMSCs. SMOC1 is highly expressed and secreted in BMSCs stimulated with osteogenic medium (OSM). SMOC1 and SMOC2 are matricellular proteins thought to influence growth factor signaling, migration, proliferation, and angiogenesis. SMOC1 and SMOC2 may mediate intercellular signaling and cell type-specific differentiation during gonad and reproductive tract development.

參考資料
  • Vannahme C. et al., 2002 J. Biol. Chem. 277:37977-86.
  • Pazin,D.E. et al., 2009, Dev Dyn. 238 (11): 2877-90.
  • Choi,Y.A. et al., 2010, J Proteome Res. 9 (6):2946-56.
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    貨號: HG12026-UT
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