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人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤

    產品資料評論相關產品實驗方法
     DIABLO cDNA Clone產品資訊
    註冊碼:NM_019887.3
    cDNA基因長度:720bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens diablo homolog (Drosophila), nuclear gene encoding mitochondrial protein, transcript variant 1 with C terminal Flag tag.
    基因別名:SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049
    分子種屬:Human
    載體:pCMV3-C-FLAG
    Plasmid:
    限制性酶切位點:
    Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    序列資訊:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with DIABLO qPCR primers for gene expression analysis, HP100033 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤 on other vectors
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10339-ACGNT$8150
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10339-ACRNT$8150
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG10339-ANGNT$8150
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG10339-ANRNT$8150
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10339-CFNT$7060
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10339-CHNT$7060
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10339-CMNT$7060
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10339-CYNT$7060
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF(克隆載體)HG10339-MNT$2720
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10339-M-FNT$7060
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10339-NFNT$7060
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10339-NHNT$7060
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10339-NMNT$7060
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10339-NYNT$7060
    人 SMAC/Diablo transcript variant 1 基因全長cDNA ORF克隆 (表達載體)HG10339-UTNT$7060
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    研究背景

    Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.

    參考資料
  • Korga A, et al. (2006) Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways Pol Merkur Lekarski. 20(119): 573-6.
  • Anguiano-Hernandez YM, et al. (2007) Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. 7(4): 467-73.
  • Martinez-Ruiz G, et al. (2008) Role of Smac/DIABLO in cancer progression. J Exp Clin Cancer Res. 27: 48.
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