|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11145-ACG|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11145-ACR|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||HG11145-ANG|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11145-CF|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11145-CH|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11145-CM|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11145-CY|
|人 S100A9/MRP-14 基因全長cDNA ORF(克隆載體)||HG11145-M|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11145-NF|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11145-NH|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11145-NM|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11145-NY|
|人 S100A9/MRP-14 基因全長cDNA ORF克隆 (表達載體)||HG11145-UT|
S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A9, also known as S100 calcium-binding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.