|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG12086-ACG|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG12086-ACR|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG12086-CF|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG12086-CH|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG12086-CM|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG12086-CY|
|人 RGMA 基因全長cDNA ORF(克隆載體)||HG12086-G|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG12086-NF|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG12086-NH|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG12086-NM|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG12086-NY|
|人 RGMA 基因全長cDNA ORF克隆 (表達載體)||HG12086-UT|
RGMa, also known as RGM domain family, member A, belongs to the RGM (repulsive guidance molecule) family whose members are membrane-associated glycoprotein. RGMa is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. It helps guide Retinal Ganglion Cell (RGC) axons to the tectum in the midbrain. RGMa has been implicated to play an important role in the developing brain and in the scar tissue that forms after a brain injury. This protein may also function as a tumor suppressor in some cancers.