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人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), C-His 標籤

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Human RELA cDNA Clone產品資訊
註冊碼:NM_021975
cDNA基因長度:1656bp
cDNA基因描述:Full length Clone DNA of Homo sapiens v-rel reticuloendotheliosis viral oncogene homolog A (avian) with C terminal His tag.
基因別名:p65
分子種屬:Human
載體:pCMV3-C-His
Plasmid:pCMV3-RELA-His
限制性酶切位點:KpnI + XbaI (6kb + 1.7kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human RELA Gene Plasmid Map
Human RELA / Transcription factor p65 / NFkB p65 ORF mammalian expression plasmid, C-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), C-His 標籤 on other vectors
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG12054-ACGNT$8880
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG12054-ACRNT$8880
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG12054-ANGNT$8880
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG12054-ANRNT$8880
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG12054-CFNT$7790
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG12054-CHNT$7790
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG12054-CMNT$7790
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG12054-CYNT$7790
人 RELA / Transcription factor p65 基因全長cDNA ORF(克隆載體)HG12054-GNT$2720
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG12054-NFNT$7790
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG12054-NHNT$7790
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG12054-NMNT$7790
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG12054-NYNT$7790
人 RELA / Transcription factor p65 基因全長cDNA ORF克隆 (表達載體)HG12054-UTNT$7790
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研究背景

RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation.

參考資料
  • Hashimoto R, et al. (2011) Variants of the RELA gene are associated with schizophrenia and their startle responses. Neuropsychopharmacology. 36(9): 1921-31.
  • Vallabhapurapu S, et al. (2009) Regulation and function of NF-kappaB transcription factors in the immune system. Annu Rev Immunol. 27: 693-733.
  • Schmitz ML, et al. (2004) NF-kappaB: a multifaceted transcription factor regulated at several levels. Chembiochem. 5(10): 1348-58.
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    貨號: HG12054-CH
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    • Human RELA / Transcription factor p65 / NFkB p65 ORF mammalian expression plasmid, C-His tag
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