|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human REG1A ORF mammalian expression plasmid, C-GFPSpark tag||HG11234-ACG|
|Human REG1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11234-ACR|
|Human REG1A ORF mammalian expression plasmid, C-Flag tag||HG11234-CF|
|Human REG1A ORF mammalian expression plasmid, C-His tag||HG11234-CH|
|Human REG1A ORF mammalian expression plasmid, C-Myc tag||HG11234-CM|
|Human REG1A ORF mammalian expression plasmid, C-HA tag||HG11234-CY|
|人 REG1A 基因全长ORF克隆||HG11234-M|
|Human REG1A ORF mammalian expression plasmid, N-Flag tag||HG11234-NF|
|Human REG1A ORF mammalian expression plasmid, N-His tag||HG11234-NH|
|Human REG1A ORF mammalian expression plasmid, N-Myc tag||HG11234-NM|
|Human REG1A ORF mammalian expression plasmid, N-HA tag||HG11234-NY|
|Human REG1A natural ORF mammalian expression plasmid||HG11234-UT|
Regenerating (reg) gene encodes protein that has been involved in pancreatic lithogenesis and the regeneration of islet cells and therefore the abnormality of reg genes could be associated with fibrocalculous pancreatopathy. REG I has been shown to be crucial for induction of ductal epithelial cells to differentiate into some cells. Lithostathine-1-alpha, also known as Pancreatic stone protein, Pancreatic thread protein, Regenerating islet-derived protein 1-alpha, REG1A, REG-1-alpha, and PSPS, is highly expressed in fetal and infant brains. REG1A contains one C-type lectin domain and is a known growth factor affecting pancreatic islet beta cells. REG1A may act as an inhibitor of spontaneous calcium carbonate precipitation. It may also be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. REG1A has been reported to be expressed in human cancers, and it may be positively correlated with patient's prognosis. REG3A and REG1A proteins are both involved in liver and pancreatic regeneration and proliferation. High levels of REG1A expression by tumor cells are an independent predictor of a poor prognosis in patients with non-small cell lung cancer (NSCLC).