|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG13533-ACG|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG13533-ACR|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG13533-CF|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG13533-CH|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG13533-CM|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG13533-CY|
|人 RCN3 基因全長cDNA ORF(克隆載體)||HG13533-G|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG13533-NF|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG13533-NH|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG13533-NM|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG13533-NY|
|人 RCN3 基因全長cDNA ORF克隆 (表達載體)||HG13533-UT|
RCN3 belongs to the CREC family which contains multiple EF-hand Ca2+-binding proteins localized to the secretory pathway. RCN3 sequence is characterized by the presence of five Arg-Xaa-Xaa-Arg motifs, which represents the target sequence of subtilisin-like proprotein convertases(SPCs). SPCs are a family of seven structurally related serine endoproteases that are involved in the proteolytic activation of proproteins.RCN3 is transiently associated with proPACE4, but not with mature PACE4. Inhibition of PACE4 maturation by a Ca2+ ionophore resulted in accumulation of the proPACE4-RCN-3 complex in cells. It has been proposed that elective and transient association of RCN3 with the precursor of PACE4 plays an important role in the biosynthesis of PACE4.