|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG13320-ACG|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG13320-ACR|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG13320-CF|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG13320-CH|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG13320-CM|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG13320-CY|
|人 Epcr/PROCR 基因全長cDNA ORF(克隆載體)||HG13320-G|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG13320-NF|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG13320-NH|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG13320-NM|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG13320-NY|
|人 Epcr/PROCR 基因全長cDNA ORF克隆 (表達載體)||HG13320-UT|
Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor) or PROCR, is a receptor for Protein C. Protein C plays an important role in many metabolism processes in humans and other animals after activated by binding to Endothelial protein C receptor (EPCR). Because of the EPCR is found primarily on endothelial cells (cells on the inside of blood vessels), activated protein C is found maily near endothelial cells. Protein C is pleiotropic, with two main functions: anticoagulation and cytoprotection. Which function will be performed depend on whether or not protein C remains bind to EPCR after activated. The anticoagulation occurs when it does not. In this case, protein C functions as an anticoagulant by irreversibly proteolytically inactivating Factor Va and Factor VIIIa, turning them into Factor Vi and Factor VIIIi respectively. When still bound to EPCR, activated protein C performs its cytoprotective effects, acting on the effector substrate PAR-1, protease-activated receptor-1. To a degree, APC's anticoagulant properties are independent of its cytoprotective ones, in that expression of one pathway is not affected by the existence of the other.