输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!

快速訂購

Text Size:AAA

人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤

產品資料評論相關產品實驗方法
Human PMVK cDNA Clone產品資訊
註冊碼:BC006089
cDNA基因長度:579bp
cDNA基因描述:Full length Clone DNA of Homo sapiens phosphomevalonate kinase with N terminal Flag tag.
基因別名:PMK, PMKA, PMKASE, HUMPMKI
分子種屬:Human
載體:pCMV3-N-FLAG
Plasmid:
限制性酶切位點:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤 on other vectors
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG14583-ACGNT$8150
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG14583-ACRNT$8150
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG14583-ANGNT$8150
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG14583-ANRNT$8150
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG14583-CFNT$7060
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG14583-CHNT$7060
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG14583-CMNT$7060
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG14583-CYNT$7060
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF(克隆載體)HG14583-GNT$2720
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG14583-NFNT$7060
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG14583-NHNT$7060
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG14583-NMNT$7060
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG14583-NYNT$7060
人 PMVK / phosphomevalonate kinase 基因全長cDNA ORF克隆 (表達載體)HG14583-UTNT$7060
 點擊瞭解更多載體資訊
Product nameProduct name
研究背景

PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.

Size / Price
貨號: HG14583-NF
目錄價: 
單價:      (You Save: )
規格:
數量:+-
庫存2-3 weeks
大包裝查詢加入購物車
Contact Us
產品圖片
      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”