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人 PLA2G2A 基因全長cDNA ORF克隆 (表達載體)

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Human PLA2G2A cDNA Clone產品資訊
註冊碼:NM_000300.2
cDNA基因長度:435bp
cDNA基因描述:Full length Clone DNA of Homo sapiens phospholipase A2, group IIA (platelets, synovial fluid).
基因別名:MOM1, PLA2, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2, PLA2G2A
分子種屬:Human
載體:pCMV3-untagged
Plasmid:pCMV3-PLA2G2A
限制性酶切位點:KpnI + XbaI (6.1kb + 0.44kb)
Tag序列:
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutation 96 G/C not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human PLA2G2A Gene Plasmid Map
Human PLA2G2A Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
Product nameProduct name
研究背景

Phospholipase A2, membrane associated, also known as Phosphatidylcholine 2-acylhydrolase 2A, Group IIA phospholipase A2, Non-pancreatic secretory phospholipase A2 and PLA2G2A, is a peripheral membrane protein which belongs to the phospholipase A2 family. PLA2G2A is found in many cells and also extracellularly. The membrane-bound and secreted forms of PLA2G2A are identical. PLA2G2A has been proposed to play a role in anti-bacterial defense, inflammation and eicosanoid generation, in clearance of apoptotic cells, and in the Wnt signaling pathway. PLA2G2A is thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. PLA2G2A catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. PLA2G2A might be a factor in human colorectal tumorigenesis.

參考資料
  • Praml,C. et al., 1998, Oncogene. 17 (15):2009-12.
  • Fijneman,R.J. et al., 2008,Front Biosci 13 :4144-74.
  • Fijneman,R.J. et al., 2009, Cell Oncol  31 (5):345-56.
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    貨號: HG11187-UT
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    • Human PLA2G2A Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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