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人 PGK1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤

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Human PGK1 cDNA Clone產品資訊
註冊碼:NM_000291.3
cDNA基因長度:1254bp
cDNA基因描述:Full length Clone DNA of Homo sapiens phosphoglycerate kinase 1 with C terminal His tag.
基因別名:PGKA, MIG10, MGC8947, MGC117307, MGC142128, PGK1
分子種屬:Human
載體:pCMV3-C-His
Plasmid:
限制性酶切位點:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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參考資料
  • Rush J. et al., 2005, Nat Biotechnol. 23: 94-101.
  • Olsen JV. et al., 2006, Cell. 127: 635-648.
  • Zieker D. et al., 2008, Cell Physiol Biochem. 21 (5-6): 429-36.
  • Jung Y. et al., 2009, Mol Cancer Res. 7 (10): 1595-604.
  • Choudhary C. et al., 2009, Science. 325: 834-40.
  • Size / Price
    貨號: HG11114-CH
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