|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10556-ACG|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10556-ACR|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10556-CF|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10556-CH|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10556-CM|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10556-CY|
|人 PDGFRA/CD140a 基因全長cDNA ORF(克隆載體)||HG10556-M|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10556-M-Y|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10556-NF|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10556-NH|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10556-NM|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10556-NY|
|人 PDGFRA/CD140a 基因全長cDNA ORF克隆 (表達載體)||HG10556-UT|
PDGFRA, also known as CD140a, together with the structurally homolog protein PDGFRB (CD140b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development. Additionally, PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.