|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11222-ACG|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11222-ACR|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||HG11222-ANG|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤||HG11222-ANR|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11222-CF|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11222-CH|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11222-CM|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11222-CY|
|人 NTMT1 Gene cDNA clone plasmid||HG11222-M|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11222-NF|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11222-NH|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11222-NM|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11222-NY|
|人 METTL11A 基因全長cDNA ORF克隆 (表達載體)||HG11222-UT|
Methyltransferase-like protein 11A, also known as METTL11A, is a member of the methyltransferase superfamily and METTL11 family. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Methylation often occurs on nucleic bases in DNA or amino acids in protein structures. Methytransferase uses a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. This methylation occurs on cytosine residues. DNA methylation may be necessary for normal growth from embryonic stages in mammals. Methylation can serve to protect DNA from enzymatic cleavage, since restriction enzymes are unable to bind and recognize externally modified sequences. This is especially useful in bacterial restriction modification systems which use restriction enzymes to cleave foreign DNA while keeping their own DNA protected by methylation. Methylation of amino acids in the formation of proteins leads to more diversity of possible amino acids and therefore more diversity of function. The methylation reaction occurs on nitrogen atoms either on the N terminus or side-chain position of the protein and are usually irreversible.