输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!

快速訂購

Text Size:AAA

人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體)

產品資料評論相關產品實驗方法
Human NEK3 cDNA Clone產品資訊
註冊碼:NM_152720.1
cDNA基因長度:1521bp
cDNA基因描述:Full length Clone DNA of Homo sapiens NIMA (never in mitosis gene a)-related kinase 3, transcript variant 2.
基因別名:HSPK36, MGC29949, NEK3
分子種屬:Human
載體:pCMV3-untagged
Plasmid:pCMV3-NEK3
限制性酶切位點:KpnI + XbaI (6.1kb + 1.52kb)
Tag序列:
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutation 1236T/G not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human NEK3 Gene Plasmid Map
Human NEK3 transcript variant 2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體) on other vectors
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤HG10675-ACGNT$8880
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤HG10675-ACRNT$8880
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤HG10675-ANGNT$8880
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤HG10675-ANRNT$8880
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10675-CFNT$7790
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤HG10675-CHNT$7790
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤HG10675-CMNT$7790
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤HG10675-CYNT$7790
人 NEK3 transcript variant 2 基因全長cDNA ORF(克隆載體)HG10675-MNT$2720
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤HG10675-M-FNT$7790
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤HG10675-NFNT$7790
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤HG10675-NHNT$7790
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤HG10675-NMNT$7790
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤HG10675-NYNT$7790
人 NEK3 transcript variant 2 基因全長cDNA ORF克隆 (表達載體)HG10675-UTNT$7790
 點擊瞭解更多載體資訊
Product nameProduct name
研究背景

NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a). NEK3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for NEK3 gene. NEK3 may play a role in mitotic regulation.

參考資料
  • Miller SL, et al. (2007) Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Oncogene. 26(32):4668-78.
  • Hernandez M, et al. (2006) Is there any association between nek3 and cancers with frequent 13q14 deletion?. Cancer Invest. 24(7):682-8.
  • Tanaka K, et al. (1999) Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators. J Biol Chem. 274(19):13491-7.
  • Size / Price
    貨號: HG10675-UT
    目錄價: 
    單價:      (You Save: )
    規格:
    數量:+-
    庫存In Stock
    大包裝查詢加入購物車
    Contact Us
    產品圖片
    • Human NEK3 transcript variant 2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”