|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG11183-ACG|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG11183-ACR|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG11183-CF|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG11183-CH|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG11183-CM|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG11183-CY|
|人 Nicastrin/NCSTN 基因全長cDNA ORF(克隆載體)||HG11183-M|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG11183-NF|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG11183-NH|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG11183-NM|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG11183-NY|
|人 Nicastrin/NCSTN 基因全長cDNA ORF克隆 (表達載體)||HG11183-UT|
Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.