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人 Alpha-galactosidase B 基因全長cDNA ORF克隆 (表達載體), N-His 標籤

  • Human CNDP1 Gene Plasmid Map 5655
產品資料評論相關產品實驗方法
 NAGA cDNA Clone產品資訊
註冊碼:NM_000262.2
cDNA基因長度:1278 bp
cDNA基因描述:Full length Clone DNA of Homo sapiens N-acetylgalactosaminidase, alpha with N terminal His tag.
基因別名:GALB, D22S674, NAGA
分子種屬:Human
載體:pCMV3-SP-N-His
Plasmid:pCMV3-SP-His-NAGA
限制性酶切位點:HindIII + NotI(6kb+1.28kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 321G/A not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with NAGA qPCR primers for gene expression analysis, HP102365 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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貨號: HG13686-NH
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Datasheet & Documentation

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