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人 MIPOL1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤

    產品資料評論相關產品實驗方法
     MIPOL1 cDNA Clone產品資訊
    註冊碼:BC035870
    cDNA基因長度:786bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens mirror-image polydactyly 1 with C terminal HA tag.
    基因別名:MIPOL1
    分子種屬:Human
    載體:pCMV3-C-HA
    Plasmid:
    限制性酶切位點:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列資訊:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with MIPOL1 qPCR primers for gene expression analysis, HP103785 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

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    貨號: HG15158-CY
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    Datasheet & Documentation

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