( We provide with OLR1 qPCR primers for gene expression analysis, HP100588 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10585-ACG|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10585-ACR|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10585-CF|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10585-CH|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10585-CM|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10585-CY|
|人 OLR1/LOX1 基因全長cDNA ORF(克隆載體)||HG10585-M|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體)||HG10585-M-N|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10585-NF|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10585-NH|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10585-NM|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10585-NY|
|人 OLR1/LOX1 基因全長cDNA ORF克隆 (表達載體)||HG10585-UT|
Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1 or OLR1), also known as lectin-type oxidized LDL receptor 1 (LOX1), is a receptor protein that belongs to the C-type lectin superfamily. LOX1 is a multi-ligand receptor originally identified as the endothelial oxidized LDL receptor. OLR1 / LOX1 was isolated from an aortic endothelial cell, and recently it has been discovered in macrophages and vascular smooth muscle cells in artery vessels. The expression of LOX1 is inducted by inflammatory stimuli and oxidative stimuli. This protein binds, internalizes and degrades oxidized low-density lipoprotein. LOX1 may play an important role in the progression of vulnerable carotid plaque and might regulate vulnerable plaque formation in cooperation with MMPs and TIMP-2. In clinical, LOX1 is thought to be involved in the development of atherosclerotic lesions.