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人 LIPG 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤

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Human LIPG cDNA Clone產品資訊
註冊碼:BC060825
cDNA基因長度:1503bp
cDNA基因描述:Full length Clone DNA of Homo sapiens lipase, endothelial with N terminal Myc tag.
基因別名:EL, EDL, PRO719, LIPG
分子種屬:Human
載體:pCMV3-SP-N-Myc
Plasmid:pCMV3-Myc-LIPG
限制性酶切位點:KpnI + XbaI (6kb + 1.54kb)
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human LIPG Gene Plasmid Map
Human LIPG ORF mammalian expression plasmid, N-Myc tag
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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貨號: HG13734-NM
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