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人 Junctional Adhesion Molecule A / JAM-A ELISA Pair Set

  • Human Junctional Adhesion Molecule A / JAM-A ELISA Pair Set
產品資料評論相關產品實驗方法
 F11R 組成成分
Capture Ab:0.5 mg/mL of mouse anti-Human Junctional Adhesion Molecule A / JAM-A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10198-MM08)
檢測抗體:0.1 mg/mL of mouse anti-Human Junctional Adhesion Molecule A / JAM-A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.4 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10198-MM02)
標準品:Each vial contains 10 ng of recombinant Human Junctional Adhesion Molecule A / JAM-A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
 F11R 檢測靈敏度
The minimum detectable dose of Human Junctional Adhesion Molecule A / JAM-A was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
 F11R 產品介紹
The Human Junctional Adhesion Molecule A / JAM-A ELISA Pair Set is for the quantitative determination of Human Junctional Adhesion Molecule A / JAM-A.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Junctional Adhesion Molecule A / JAM-A coated on a 96-well plate. Standards and samples are added to the wells, and any Human Junctional Adhesion Molecule A / JAM-A present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human Junctional Adhesion Molecule A / JAM-A monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Junctional Adhesion Molecule A / JAM-A present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
 F11R 儲存方法
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
 F11R ELISA Pair Set Standard Curve
Junctional Adhesion Molecule A 研究背景

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.

 Junctional Adhesion Molecule A 參考資料
  • Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.
  • Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.
  • Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
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    貨號: SEKA10198-5
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