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人 ISG20L2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤

  • Human aFGF/FGF1 Gene Plasmid Map 5637
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 ISG20L2 cDNA Clone產品資訊
註冊碼:NM_030980.1
cDNA基因長度:1101 bp
cDNA基因描述:Full length Clone DNA of Homo sapiens interferon stimulated exonuclease gene 20kDa-like 2 with N terminal Flag tag.
基因別名:ISG20L2
分子種屬:Human
載體:pCMV3-N-FLAG
Plasmid:pCMV3-Flag-ISG20L2
限制性酶切位點:KpnI + XbaI(6kb+1.1kb)
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 228G/A,870A/G not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with ISG20L2 qPCR primers for gene expression analysis, HP105079 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

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