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人 IL6 / IL-6 ELISA Kit

  • Human IL6 / IL-6 ELISA Kit, TYPICAL DATA
  • Human IL6 / IL-6 ELISA Kit, SPECIFICITY
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檢測原理
Solid Phase Sandwich ELISA
預期用途
For the quantitative determination of Human IL6 / IL-6 concentration in cell culture supernates.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
線性範圍
18.75-1200 pg/mL
靈敏度
The minimum detectable dose of Human IL6 / IL-6 is typically less than 10.76 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
試劑盒組分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
儲存方法
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
 IL6 ELISA Kit Standard Curve
Human IL6 / IL-6 ELISA Kit, TYPICAL DATA
[點擊查看大圖]
Caption:
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
 IL6 ELISA Kit Specificity
Human IL6 / IL-6 ELISA Kit, SPECIFICITY
[點擊查看大圖]
Caption:
This assay recognizes both recombinant and natural Human IL6. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL6/IL-6/Interleukin-6 研究背景

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

 IL6/IL-6/Interleukin-6 參考資料
  • Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  • Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  • Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
  • Size / Price
    貨號: KIT10395-1
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”