IL-6 ELISA Kit, Human

Cat: KIT10395

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IL-6 ELISA Kit, Human General Information
Product name
IL-6 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Detection antibody conjugate
Sample type
Cell supernatant
10.76 pg/mL
Assay range
18.75-1200 pg/mL
Recognizes both recombinant and natural Human IL-6
Preparations of the following factors at 100 ng/mL in a mid-range recombinant human IL6 control were assayed for interference. No significant interference was observed.
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-6 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-6 protein in Cell supernatant . It contains recombinant Human IL-6, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
This ELISA Kit is shipped at ambient temperature.
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
IL-6 ELISA Kit, Human Images
Human IL6/IL-6/Interleukin-6 ELISA Kit Standard Curve 62
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human IL6/IL-6/Interleukin-6 ELISA Kit Specificity 211
This assay recognizes both recombinant and natural Human IL6. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL-6 ELISA Kit, Human Alternative Names
BSF2 ELISA Kit, Human;HGF ELISA Kit, Human;HSF ELISA Kit, Human;IFNB2 ELISA Kit, Human;IL-6 ELISA Kit, Human;Interleukin-6 ELISA Kit, Human
IL-6 Background Information

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
interleukin 6
  • Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  • Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  • Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    IL-6 ELISA Kit, Human Serum,Cell supernatant KIT10395A
    IL-6 Matched ELISA Antibody Pair Set,Human 2.34 pg/ml SEKB10395
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