|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10607-ACG|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10607-ACR|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10607-CF|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10607-CH|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10607-CM|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10607-CY|
|人 IL-1F6 基因全長cDNA ORF(克隆載體)||HG10607-M|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10607-NF|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10607-NH|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10607-NM|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10607-NY|
|人 IL-1F6 基因全長cDNA ORF克隆 (表達載體)||HG10607-UT|
Interleukin-1 family member 6 (IL-1F6), also known as interleukin 36, alpha (IL36A), is a pro-inflammatory cytokine which plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta, suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line, NCI/ADR-RES, which naturally expresses IL-1Rrp2, all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6, IL-1F8, and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.