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人 IL1RL1/IL‑1 R4 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤

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Human IL1RL1 cDNA Clone產品資訊
註冊碼:NM_003856.2
cDNA基因長度:987bp
cDNA基因描述:Full length Clone DNA of Homo sapiens interleukin 1 receptor-like 1, transcript variant 2 with N terminal His tag.
基因別名:IL1RL1, T1, ST2, DER4, ST2L, ST2V, FIT-1, MGC32623
分子種屬:Human
載體:pCMV3-SP-N-His
Plasmid:pCMV3-His-IL1RL1
限制性酶切位點:KpnI + NotI (6kb + 1.03kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human IL1RL1 Gene Plasmid Map
Human IL1RL1 / ST2 transcript variant 2 natural ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

人 IL1RL1/IL‑1 R4 transcript variant 2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤 on other vectors
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研究背景

IL-1 receptor–like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interlukin-1 like 1 (IL1RL1) protein can be used to diagnose cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described, and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage pro-inflammatory function. IL33 is a specific ligand of ST2L and induces production of Th2 cytokines.

參考資料
  • Savenije OE, et al. (2011) Interleukin-1 receptor-like 1 polymorphisms are associated with serum IL1RL1-a, eosinophils, and asthma in childhood. J Allergy Clin Immunol. 127(3): 750-6.
  • Li H, et al. (2000) The cloning and nucleotide sequence of human ST2L cDNA. Genomics. 67(3): 284-90.
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    貨號: HG10105-NH
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    • Human IL1RL1 / ST2 transcript variant 2 natural ORF mammalian expression plasmid, N-His tag
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