IL4R ELISA Kit, Human

Cat: KIT10402
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IL4R ELISA Kit, Human General Information
Product name
IL4R ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant
Sensitivity
2.6 pg/mL
Assay range
3.125-200 pg/mL
Specificity
Recognizes both recombinant and natural Human IL4R
Interference
Preparations of human IL4 at 2 ng/mL in a mid-range recombinant human IL4R control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 42.90 76.50 140.80
SD 1.81 4.88 7.68
CV(%) 4.2% 6.4% 5.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 39.30 75.70 143.50
SD 2.40 3.53 9.12
CV(%) 6.1% 4.7% 6.4%
Recovery
The recovery of Human IL4R spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 95 89 -97%
supernatant (n=3) 91 87 -96%
Linearity
Serum
  Recovery of detected
1:2 80%
1:4 102%
1:8 99%
1:16 99%
Supernatant
  Recovery of detected
1:2 100%
1:4 101%
1:8 105%
1:16 98%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL4R ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL4R protein in Serum,Cell supernatant . It contains recombinant Human IL4R, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
IL4R ELISA Kit, Human Images
Human IL-4R/CD124 ELISA Kit Standard Curve 63
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human IL-4R/CD124 ELISA Kit Specificity 212
This assay recognizes both recombinant and natural Human IL4R. The factors listed below were prepared at 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL4R ELISA Kit, Human Alternative Names
CD124 ELISA Kit, Human;IL-4RA ELISA Kit, Human;IL4R ELISA Kit, Human;IL4RA ELISA Kit, Human
IL4R Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a typeⅠ transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.

Full Name
interleukin 4 receptor
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
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